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Isolation and characterization of a 7 S RNP particle from mature Xenopus laevis oocytes
Author(s) -
Pieler Tomas,
Erdmann Volker A.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80562-6
Subject(s) - xenopus , ribosomal rna , rna , biology , ribonucleoprotein , ribonucleoprotein particle , microbiology and biotechnology , genetics , gene
Mature oocytes of Xenopus laevis contain a 7 S RNP particle consisting of two components, ribosomal 5 S RNA and a protein of M r ≈45 000. The structure of the free 5 S rRNA and the 7 S RNP complex has been studied by diethylpyrocarbonate modification of adenines. A 74 , A 77 , A 90 , A 100 and A 103 of the 5 S rRNA are protected upon association of the protein.