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Carbohydrate structures of hen ovomucoid
Author(s) -
Egge Heinz,
Peter-Katalinić Jasna,
Paz-Parente José,
Strecker Gerard,
Montreuil Jean,
Fournet Bernard
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80528-6
Subject(s) - chemistry , glycan , mass spectrometry , fast atom bombardment , carbohydrate , hydrolysis , mass spectrum , chromatography , acid hydrolysis , nuclear magnetic resonance spectroscopy , analytical chemistry (journal) , organic chemistry , biochemistry , glycoprotein
The apparently homogenous N ‐glycosidically‐linked glycans 1, 7, 11 and 14 released by hydrazinolysis from hen ovomucoid were analysed by fast atom bombardment and electron‐impact mass spectrometry after reduction and permethylation. The spectra support the primary structures established independently [FEBS Letters (1983) 152, 145–152] using methylation analysis, partial acid hydrolysis and 500 MHz 1 H NMR spectroscopy. In addition to the major constituents present in fractions 1, 7, 11 and 14, four minor components not detected by other methods could be characterized with the aid of the mass spectrometry data as: Man 2 GlcNAcGlcNAc‐ol, GlcNAc 4 Man 3 GlcNAc‐ol, GlcNAc 6 Man 3 GlcNAc‐ol and GalGlcNAc 6 Man 3 GlcNAc‐ol. Our results show that the physical techniques used provide valuable data on the structure of complex glycans. In addition they can be employed to ascertain the homogeneity of the compounds examined as well as to detect trace amounts of homologs that might not be noticed by other methods.