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The TolC protein of Escherichia coli K12 is synthesised in a precursor form
Author(s) -
Hackett Jim,
Misra Rajeev,
Reeves Peter
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80518-3
Subject(s) - escherichia coli , immunoprecipitation , sequence (biology) , microbiology and biotechnology , biosynthesis , signal peptide , biology , enterobacteriaceae , protein biosynthesis , gene , peptide sequence , chemistry , biochemistry
We examined the biosynthesis of the TolC protein of Escherichia coli K12 in a pulse—chase experiment, followed by immunoprecipitation with anti‐TolC antibody and SDS—PAGE of the immunoprecipitate. This showed that TolC protein was originally synthesised in a precusor form ( M r 54 500) which could be chased into the mature form ( M r 52 000). DNA sequencing of a portion of the cloned tolC gene showed that the N‐terminus of the mature rotein was preceded by a typical signal sequence of 22 residues ( M r 2542). The initiator Met was preceded by a Shine—Dalgarno sequence, with the correct spacing.