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Simultaneous isolation of the yeast cytosol and well‐preserved mitochondria with negligible contamination by vacuolar proteinases
Author(s) -
Schwencke Jaime,
Canut Hervé,
Flores Alberto
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80512-2
Subject(s) - vacuole , spheroplast , cytosol , yeast , isolation (microbiology) , mitochondrion , contamination , biochemistry , cell fractionation , biology , chemistry , enzyme , microbiology and biotechnology , cytoplasm , ecology , escherichia coli , gene
Disruption of yeast spheroplasts by DEAE‐dextran in isoosmotic conditions allows isolation of relatively undamaged subcellular fractions from yeast. The preservation of mitochondria and vacuoles permits the simultaneous isolation of the cytosol with negligible contamination by vacuolar proteinases and therefore, virtually eliminates proteolytic artefacts.