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The amino acid sequence of the α‐subunit of a mitogenic lectin from seeds of Lathyrus odoratus
Author(s) -
Sletten Knut,
Kolberg Jan,
Michaelsen Terje E.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80507-9
Subject(s) - edman degradation , lathyrus , biochemistry , lectin , peptide sequence , pisum , amino acid , biology , chemistry , botany , gene
We have determined the complete amino acid sequence (54 residues) of the light chain of the mitogenic, glucose‐ and mannose‐specific lectin from the seeds of Lathyrus odoratus . The covalent structure was elucidated by Edman degradation on the whole chain as well as on fragments derived from cleaving the chain with BNPS‐skatole and of peptides derived after tryptic digestion of the citraconylated chain. The amino acid sequence revealed a high homology to the light chains of lectins from Pisum sativum and Lens culinaris .