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Norepinephrine‐induced loss of phosphatidylinositol from isolated rat liver plasma membrane
Author(s) -
Wallace Michael A.,
Poggioli Josiane,
Giraud Françoise,
Claret Michel
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80504-3
Subject(s) - phosphatidylinositol , chemistry , membrane , phentolamine , divalent , norepinephrine , incubation , endocrinology , antagonist , medicine , biophysics , receptor , biochemistry , biology , signal transduction , dopamine , organic chemistry
Norepinephrine at 5 μM induces a rapid (60 s) and specific loss of phosphatidylinositol (PtdIns) when added to isolated rat liver plasma membranes. The hormone action is inhibited by the α‐adrenergic antagonist phentolamine (20 μM). Depletion of Mg 2+ and Ca 2+ singly or in combination from the incubation buffer mimicks the hormone effect on PtdIns breakdown. No further effect on PtdIns degradation could be measured when norepinephrine was added to the cation‐depleted buffers. Addition of the Ca 2+ ionophore A23187 to the isolated membranes has no effect. It is concluded that PtdIns degradation can be provoked in isolated rat liver plasma membrane through α‐adrenergic receptor activation and that this effect is dependent on divalent cations in the sense that loss of cations from the membrane allows degradation to commence.