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Microsomal Ca 2+ ‐ and phospholipid‐dependent protein kinase
Author(s) -
Sommarin Marianne,
Jergil Bengt
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80502-x
Subject(s) - microsome , phospholipid , cytosol , enzyme , biochemistry , microsoma , egta , chemistry , protein kinase a , membrane , biology , calcium , organic chemistry
A Ca 2+ ‐ and phospholipid‐dependent protein kinase (CaPK) has been identified in rat liver microsomes. CaPK isolated from liver cytosol bound to smooth microsomes in the presence of 100 μM CaCl 2 . A saturation in binding was observed when a 5‐fold excess of enzyme over that present in microsomes had become bound. The microsomal CaPK and 50% of the enzyme bound in vitro was not removed by EGTA treatment. This suggests that Ca 2+ is required for the binding of CaPK to microsomes, but not for the retention of the enzyme on the membrane.