Premium
Intracellular pH topography: determination by a fluorescent probe
Author(s) -
Slavík Jan
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80501-8
Subject(s) - chemistry , intracellular ph , fluorescence , vacuole , cytoplasm , intracellular , biophysics , yeast , phase (matter) , chromatography , analytical chemistry (journal) , biochemistry , biology , optics , physics , organic chemistry
The distribution of pH inside the yeast Endomyces magnusii was measured at 1 μm resolution using different external pH values. In a neutral buffer the pH of the cytoplasm was 6.7–7.2 in the center, decreasing to 6.0 toward the periphery of the cell. A decrease of external pH was followed by a gradual uniform decrease of internal pH. Using a comparison of the ‘pH map’ with phase‐contrast picture of the same cell, the pH of the vacuoles was estimated to be 5.5–5.6.