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Localization of the chemotactic domain in fibronectin
Author(s) -
Albini A.,
Richter H.,
Pontz B.F.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80500-6
Subject(s) - fibronectin , chemotaxis , fibronectins , fibroblast , protein subunit , peptide , binding site , chemistry , microbiology and biotechnology , biochemistry , enzyme , biology , extracellular matrix , in vitro , receptor , gene
Fragments derived from human plasma fibronectin by enzymatic degradation were tested in the Boyden chamber for chemotactic activity towards various fibroblast strains. The results provide clear evidence that the chemotactic activity is restricted to a defined region of the fibronectin molecule which is the same for various fibroblast strains. The active domain is localized between the collagen binding site and the major heparin binding site, about 170 kDa apart from the N‐terminal and about 70 kDa from the C‐terminal ends of the two subunit peptide chains.