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Susceptibility of ribosomes of the tetracycline‐producing strain of Streptomyces aureofaciens to tetracyclines
Author(s) -
Mikulík Karel,
Jiráňová Anna,
Janda Ivan,
Weiser Jaroslav
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80496-7
Subject(s) - ribosome , streptomyces aureofaciens , tetracycline , protein biosynthesis , ribosomal rna , transfer rna , strain (injury) , tetracycline antibiotics , translation (biology) , ribosomal protein , escherichia coli , chemistry , chlortetracycline , microbiology and biotechnology , biology , biochemistry , streptomyces , antibiotics , rna , bacteria , genetics , messenger rna , gene , anatomy
Ribosomes from cells of Streptomyces aureofaciens producing tetracycline antibiotics (Tc‐ribosomes) differ in electrophoretic mobility of ribosomal proteins S2, S10 and L19 from those of the same strain, where the production of tetracyclines was suppressed by changed cultivation conditions (C‐ribosomes). Purified tight vacant couples C‐ and Tc‐ribosomes are equally active in the translation of poly(U). Both types of S. aureofaciens ribosomes are more sensitive to tetracycline and chlortetracycline than ribosomes of Escherichia coli in the Phe‐tRNA binding and the translation of poly(U).