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Differential crosslinking of histones and non‐histones in nuclei by cis‐Pt(II)
Author(s) -
Filipski Jan,
Kohn Kurt W.,
Bonner William M.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80492-x
Subject(s) - histone , thiourea , chemistry , nuclear protein , cell nucleus , chromatin , non histone protein , biochemistry , dna , cytoplasm , organic chemistry , gene , transcription factor
When nuclei were treated with the chemotherapeutic agent, cis‐Pt(II), they were crosslinked to the extent that their nuclear morphology as assayed by light microscopy was retained even in the presence of SDS. Protein analysis showed that the histones were completely absent from these nuclear structures, while the non‐histone proteins, with one possible exception, were completely retained. When the nuclear structures in SDS were treated with thiourea to reverse the crosslinks, the non‐histone proteins were liberated and the nuclear structures disappeared. When treated with Proteinase K in SDS, the nuclear structures also disappeared, indicating that protein components were necessary to maintain the structures.