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Amino acid sequence around the active site cysteine residue of calcium‐activated neutral protease (CANP)
Author(s) -
Suzuki Koichi,
Hayashi Hiroaki,
Hayashi Tomoko,
Iwai Koichi
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80483-9
Subject(s) - chemistry , cysteine , residue (chemistry) , calcium , active site , amino acid residue , biochemistry , protease , cysteine protease , peptide sequence , amino acid , stereochemistry , enzyme , organic chemistry , gene
The active site of calcium‐activated neutral protease (CANP) was specifically labeled with iodoacetic acid. The active site hepta peptide was isolated from carboxymethylated CANP after digestion with proteases and the sequence was determined. The sequence indicates that CANP is a thiol protease and its comparison with other thiol proteases is described.