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The effect of a ribonuclease inhibitor from human placenta on the in vitro synthesis of human placental proteins
Author(s) -
Hiranyavasit W.,
Kusamran T.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80476-1
Subject(s) - ribonuclease , human placental lactogen , immunoprecipitation , human placenta , placenta , polyacrylamide gel electrophoresis , biochemistry , gel electrophoresis , placental lactogen , translation (biology) , biology , antiserum , in vitro , human chorionic gonadotropin , microbiology and biotechnology , chemistry , messenger rna , enzyme , rna , fetus , gene , hormone , antibody , pregnancy , immunology , genetics
Addition of a ribonuclease inhibitor (10 μg/ml) from human placenta caused 2–3 fold increase of [ 3 H]leucine incorporation in the wheat germ extract as directed by human placental poly (A)‐mRNA. Analysis of the translated products by sodiumdodecylsulfate/polyacrylamide gel electrophoresis/fluorography revealed that the inhibitor preferentially increased the yields of the larger proteins, particularly those of larger than M r 40 000. In the presence of the inhibitor, yields of two placental proteins (human placental lactogen and human chorionic gonadotropin) were increased about 70–80% as detected by immunoprecipitation with specific homologous antisera. The method provided an improvement of translation system for studying biosynthesis of other human placental proteins.