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Reconstitution of photosynthetic electron transfer in cyanobacterial heterocyst membranes
Author(s) -
Hawkesford Malcolm J.,
Houchins Jeffrey P.,
Hind Geoffrey
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80460-8
Subject(s) - ferredoxin , plastocyanin , heterocyst , photosystem i , p700 , electron transport chain , chemistry , cytochrome b6f complex , electron transfer , photosystem , cytochrome , biochemistry , photosynthesis , hydrogenase , photochemistry , ferredoxin—nadp(+) reductase , nadh dehydrogenase , anabaena , biophysics , photosystem ii , biology , enzyme , cyanobacteria , protein subunit , bacteria , gene , genetics
Photosynthetic membranes have been isolated from heterocysts of the cyanobacterium Anabaena 7120. Electron transfer reactions associated with photosystem I have been investigated using single turnover flashes. Turnover rates, which were dependent on reconstitution with cytochrome c ‐553 or plastocyanin, were maximal when a suitable electron donor system was provided. Hydrogen (via an endogenous uptake hydrogenase), NADH (via a dehydrogenase) or the ascorbate/dichlorophenolindophenol couple were all effective donors. NADPH could also serve as a donor but this system was dependent on addition of ferredoxin and ferredoxin—NADP reductase. Turnovers of P700, soluble cytochrome c ‐553 and cytochrome b ‐563 could be demonstrated.