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In vivo 31 P‐NMR studies of myocardial high energy phosphate metabolism during anoxia and recovery
Author(s) -
Neurohr Klaus J.,
Gollin Gerald,
Barrett Eugene J.,
Shulman Robert G.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80447-5
Subject(s) - phosphocreatine , phosphate , chemistry , anoxic waters , in vivo , oxygen , nuclear magnetic resonance spectroscopy , inorganic phosphate , metabolism , energy metabolism , high energy phosphate , biochemistry , biology , endocrinology , stereochemistry , environmental chemistry , microbiology and biotechnology , organic chemistry
31 P NMR spectra of heart in‐situ in live guinea pigs were obtained continuously in 20.5 s time blocks during 3 min of anoxia, during subsequent reoxygenation and, in separate animals, during terminal anoxia. Reversible anoxia resulted in rapid degradation of phosphocreatine ( t ½ = 54.5 ± 2.5 s) which recovered fully during reoxygenation. Heart P i increased during anoxia and returned to basal levels after oxygen was restored. During 3 min of anoxia, no significant changes in ATP levels or pH were detected. The results demonstrate that it is feasible to measure rapid fluxes of high energy phosphates by 31 P NMR in intact animals during and after anoxic stress to the myocardium.