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Application of red‐edge effect on the mobility of membrane lipid polar head groups
Author(s) -
Raudino Antonio,
Guerrera Franco,
Asero Antonio,
Rizza Victor
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80413-x
Subject(s) - liposome , chemistry , phosphatidylcholine , polar , membrane , membrane fluidity , biophysics , chromatography , biochemistry , phospholipid , biology , physics , astronomy
The dynamic properties of membrane lipid polar head groups have been studied using the spectroscopic phenomenon known as the red‐edge effect (REE). Ree was measured in liposomes prepared with different composition of phospholipids and doped with a merocyanine dye. There was a gradual loss of REE with phosphatidylcholine (PC) liposomes as the temperature increased from 4–25°C. Addition of 10 −4 M Ca 2+ increased the REE at all temperatures measured. Liposomes prepared with phosphatidyl serine (PS) or GM 1 gangliosides showed a slight REE only at low temperatures (0–4°C). Cholesterol, Na + , K + or variations of pH had no influence on the REE. The data obtained indicate that increased molecular freedom or fluidity of lipid polar head groups can be correlated to a decrease in REE.