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Protection against lipid peroxidation by a microsomal glutathione‐dependent labile factor
Author(s) -
Haenen Guido R.M.M.,
Bast Aalt
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80409-8
Subject(s) - lipid peroxidation , microsome , glutathione , chemistry , biochemistry , gpx4 , oxidative stress , in vitro , enzyme , glutathione peroxidase
Glutathione (GSH) protects rat liver microsomes against ascorbic acid (0.2 mM)/ferrous iron (10 μM)‐induced lipid peroxidation for some time. The inhibitory effect of GSH is concentration‐dependent (0.1–1.0 mM). Our data suggest that GSH acts by preventing initial radical formation rather than via radical scavenging or GSH—peroxidase activity. A labile GSH‐dependent factor is involved in the inhibition of microsomal lipid peroxidation by GSH, inasmuch as heating the microsomes abolishes the GSH effect. We found that besides heating, lipid peroxidation also destroys the GSH‐dependent factor. Consequently, continuous radical stress will produce lipid peroxidation, despite the presence of GSH. Moreover, a detrimental effect of in vivo‐induced lipid peroxidation (CCl 4 ‐treatment) on the GSH‐dependent factor was observed. The implications of the present data for the genesis of and the protection against peroxidative damage are discussed.