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Tight coordination of ribonucleotide reduction and thymidylate synthesis in synchronous algae
Author(s) -
Bachmann Bernd,
Hofmann Rüdiger,
Follmann Hartmut
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80389-5
Subject(s) - thymidylate synthase , ribonucleotide reductase , ribonucleotide , ribonucleoside , dna synthesis , deoxyribonucleotide , biochemistry , chemistry , enzyme , biology , dna , nucleotide , rna , fluorouracil , oligonucleotide , genetics , protein subunit , chemotherapy , gene
Ribonucleoside diphosphate reductase and thymidylate synthase activities follow a virtually identical peak pattern during the cell cycle of Scenedesmus obliquus , coinciding with DNA synthesis. Both enzyme change in parallel when cultures are treated with inhibitors specific for one of them: 5‐fluoro‐2′‐deoxyuridine (a thymidylate synthesis inhibitor) stimulates 30‐fold, and hydroxyurea, inhibitory to eukaryotic ribonucleotide reduction, also suppresses thymidylate synthase. We conclude that the two enzymes of deoxyribonucleotide formation are subject to one common intracellular control mechanism.