Premium
Organization of ribosomal DNA in yellow lupine ( Lupinus luteus ) and sequence of the 5.8 S RNA gene
Author(s) -
Rafalski Jan Antoni,
Wiewiórowski Maciej,
Söll Dieter
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80388-3
Subject(s) - ribosomal rna , biology , genetics , gene , microbiology and biotechnology , southern blot , 5.8s ribosomal rna , ribosomal dna , rna , 28s ribosomal rna , 5s ribosomal rna , 18s ribosomal rna , ribosome , phylogenetics
The large ribosomal repeat of the Lupinus luteus genome is 8.8 kb in length as revealed by Southern blotting and hybridization to 5.8 S rRNA and cloned rDNA fragments of L. luteus and Schizosaccharomyces pombe . It codes for 18 S,5.8 S and 28 S rRNAs. A cloned 3.7 kb Eco R1 subfragment of the rDNA repeat, coding for 5.8 S rRNA and a large portion of the 28 S rRNA, has been sequenced in the region of 5.8 rRNA gene. The predicted rRNA sequence is homologous to broad bean 5.8 S rRNA and can be arranged in the generalized model of 5.8 S RNA secondary structure.