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Conformational change of mastoparan from wasp venom on binding with phospholipid membrane
Author(s) -
Higashijima Tsutomu,
Wakamatsu Kaori,
Takemitsu Mari,
Fujino Masahiko,
Nakajima Terumi,
Miyazawa Tatsuo
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80385-8
Subject(s) - mastoparan , melittin , phospholipid , venom , chemistry , conformational change , membrane , circular dichroism , aqueous solution , biophysics , stereochemistry , biochemistry , organic chemistry , biology , g protein , receptor
The conformational change upon binding with phospholipid membrane has been studied of mastoparan from wasp venom, a tetradecapeptide causing the degranulation of mast cells. The 270‐MHz 1 H‐NMR spectra and CD spectra indicate that the mastoparan molecule takes the α‐helical conformation in methanol solution, but a much less ordered form in aqueous solution. On binding with phospholipid membrane, the α‐helical conformation is formed even in aqueous medium. Such a conformational change is primarily due to the interaction between the aliphatic side chains of mastoparan and the hydrophobic interior of phospholipid membrane, in contrast to the case of melittin from bee venom.