Premium
Decreased activity of uroporphyrinogen decarboxylase caused by 2,4,5,3′,4′‐pentabromobiphenyl in chick embryo hepatocyte cultures
Author(s) -
Sinclair P.R.,
Elder G.H.,
Bement W.J.,
Smith S.G.,
Bonkowsky H.L.,
Sinclair J.F.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80383-4
Subject(s) - uroporphyrinogen iii decarboxylase , incubation , in vivo , embryo , enzyme , hepatocyte , chemistry , microbiology and biotechnology , cell culture , biochemistry , enzyme assay , specific activity , in vitro , biology , heme , genetics
Uroporphyrinogen decarboxylase activity was investigated in cultures of chick embryo liver by two different methods: (1) analysis of porphyrin composition following incubation of intact cells with δ‐aminolevulinic acid; and (2) a more conventional direct enzymic assay of cell homogenates. Activity was detectibly decreased following exposure of cells to 100 ng/ml 2,4,5,3′,4′‐pentabromobiphenyl using the first method, but not the second. This decrease in activity was reversed by homogenizing the cells treated with 100 ng/ml pentabromobiphenyl. It is concluded that the direct homogenate assay of the enzyme may miss or underestimate decreases in its in vivo activity.