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Interaction of polymerases with 2′‐deoxyuridine‐5′‐triphosphate spin‐labeled at the 5‐position
Author(s) -
Warwick-Koochaki P.E.,
Hakan A.,
Bobst A.M.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80369-x
Subject(s) - reverse transcriptase , dna polymerase , polymerase , nucleotide , chemistry , base pair , rna , dna , deoxyuridine , rna directed dna polymerase , biochemistry , microbiology and biotechnology , stereochemistry , biology , gene
2′‐Deoxyuridine‐5′‐triphosphate spin‐labeled at the 5‐position with N ‐[1‐oxyl‐2,2,6,6‐tetramethyl‐4‐piperidinyl]‐O‐ was found to be an inhibitor of some DNA and RNA polymerases including avian myeloblastosis virus reverse transcriptase. Furthermore, the spin‐labeled nucleotide was found to be incorporated internally into polydeoxythymidylic acid via reverse transcriptase to an extent of 1.0 spin‐labeled base per 10 3 bases. The incorporation, monitored by electron spin resonance, is analogous to some other nucleotide inhibitors of polymerases, and the results indicate that it may be feasible to obtain sequence specific, spin‐labeled DNA, enzymatically.