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Isolation and characterization of the Co‐methyl and Co‐aquo derivative of 5‐hydroxybenzimidazolylcobamide (factor III) from Methanosarcina barkeri grown on methanol
Author(s) -
Höllriegl Ver,
Scherer Paul,
Renz Paul
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80363-9
Subject(s) - methanosarcina barkeri , derivative (finance) , chemistry , methanosarcina , methanol , methanomicrobiales , stereochemistry , medicinal chemistry , biochemistry , organic chemistry , methane , archaea , gene , methanogenesis , financial economics , economics
The Co‐methyl and the Co‐aquo derivative of 5‐hydroxybenzimidazolylcobamide (factor III) were isolated as the major natural corrinoids from Methanosarcina barkeri ‘Fusaro’. They were characterized by their UV/VIS‐spectra, their chromatographic behaviour, and their electrophoretic properties. The portion of Co‐methyl‐factor III was found to be 70–80% whereas Co‐aquo‐factor III contributed 20–30% to the total amount of corrinoids.