Premium
Translation in vitro and regulation of mouse liver S ‐adenosylmethionine synthetase messenger RNA
Author(s) -
Sato Fumiyasu,
Kitahara Namiko,
Ohmura Nobuhisa,
Tsukada Kinji
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80361-5
Subject(s) - reticulocyte , messenger rna , microbiology and biotechnology , biology , methionine , translation (biology) , polyacrylamide gel electrophoresis , rna , protein biosynthesis , protein subunit , biochemistry , in vitro , in vivo , gel electrophoresis , immunoprecipitation , enzyme , gene , amino acid
Total RNA was isolated from adult mouse liver tissues. The α‐ and β‐form isozymes of S ‐adenosyl‐methionine synthetase existing in liver were synthesized in a reticulocyte lysate cell‐free system under the direction of total RNA and were immunoprecipitated with antibody to the β‐form. The newly synthesized and the in vivo labeled S ‐adenosylmethionine synthetase subunits were compared by SDS—polyacrylamide gel electrophoresis. Both the α‐ and β‐forms consist of the same size M r 48 000 subunit. The level of the β‐form mRNA activity in mouse liver was shown to increase following intraperitoneal transplantation of Ehrlich ascites tumor cells and the changes in the mRNA activity parallel those in the cellular level of S ‐adenosylmethionine synthetase β.