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Decrease in uH2A (protein A24) of a mouse temperature‐sensitive mutant
Author(s) -
Matsumoto Yoh-ichi,
Yasuda Hideyo,
Marunouchi Tohru,
Yamada Masa-atsu
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80359-7
Subject(s) - mutant , chemistry , enzyme , microbiology and biotechnology , biochemistry , ubiquitin , temperature sensitive mutant , degradation (telecommunications) , biophysics , biology , telecommunications , gene , computer science
ts85 cell is a temperature‐sensitive mutant of cell cycle, and chromosomal protein uH2A of this mutant disappears at the non‐permissive temperature. uH2A in nucleosomes is thought to be synthesized or degradated as follows. H2A + Ubiquitin ⇆ uH2A. Up to date, the degradation of uH2A was shown to be catalyzed by uH2A lyase, however no enzymes (factors) concerning its synthesis have been elucidated. Here, we show that ATP is prerequisite for the synthesis of uH2A, and that the disappearance of uH2A at the non‐permissive temperature may be due to a reduction in the rate of synthesis rather than an increase in the rate of its degradation.