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Identification of two discrete ribonucleoprotein particles within the monomer population of rat liver nuclear RNPs
Author(s) -
Guialis Apostolia,
Arvanitopoulou Anastasia,
Patrinou-Georgoula Meropi,
Sekeris Constant E.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80357-3
Subject(s) - ribonucleoprotein , identification (biology) , monomer , population , chemistry , biophysics , heterogeneous ribonucleoprotein particle , rna , computational biology , biology , physics , biochemistry , nuclear magnetic resonance , polymer , medicine , gene , botany , environmental health
30–50 S RNP particles (monoparticles) isolated from rate liver nuclei were submitted to electrophoresis in native 0.5% agarose gels. Two RNP fractions were thus separated, a minor one remaining closer to the top of the gel (MI) and a more abundant one migrating further into the gel (MII). SDS‐polyacrylamide gel electrophoresis revealed that MII contains the major monoparticle ( M r 30 000–40 000 or ‘core’) polypeptides and higher molecular weight proteins, whereas MI contains several minor proteins of M r >40 000. Some proteins are common to both particle classes. Urea‐acrylamide gel electrophoresis revealed that HnRNA is mainly present in MII, whereas snRNA is confined to the MI particle class.