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Hydrophobic properties of Tetrahymena calmodulin related to the phosphodiesterase activity
Author(s) -
Inagaki Masaki,
Naka Michiko,
Nozawa Yoshinori,
Hidaka Hiroyoshi
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80344-5
Subject(s) - tetrahymena , calmodulin , phosphodiesterase , chemistry , tetrahymena pyriformis , biochemistry , enzyme , biophysics , biology
We have examined hydrophobic properties of Tetrahymena CaM using the uncharged probe, n ‐phenyl‐1‐naphthylamine (NPN) fluorescence. The maximal flurorescence intensity of Tetrahymena calmodulin (CaM) is < 1/12 of the bovine brain CaM. In the phosphodiesterase activation, the potency of Tetrahymena CaM, which was represented by reciprocals of the quantity of CaM required for half‐maximal activation of enzyme was 22.7% respectively, of that of the bovine brain CaM. Here, Tetrahymena CaM had less hydrophobic groups exposed in the presence of Ca 2+ . Then Ca 2+ ‐CaM dependent enzymes require much amount of Tetrahymena CaM, comparing with the bovine brain CaM.