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Structure determination of the major asparagine‐linked sugar chain of human factor VII—von Willebrand factor
Author(s) -
Debeire P.,
Montreuil J.,
Samor B.,
Mazurier C.,
Goudemand M.,
Van Halbeek H.,
Vliegenthart J.F.G.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80334-2
Subject(s) - von willebrand factor , glycan , chemistry , asparagine , carbohydrate , biochemistry , sugar , glycoprotein , microbiology and biotechnology , platelet , immunology , biology , enzyme
N ‐glycosidically‐linked glycans released by hydrazinolysis of human factor VIII/von Willebrand factor (FVIII/vWf) were seperated by high‐voltage electrophoresis. Five fraction were obtained, one of them representing 60% of the total amount of the N ‐glycosidically‐linked glycans of FVIII/vWf. On the basis of the carbohydrate composition, methylation analysis and 500 MHz 1 H‐NMR spectroscopy, we describe the primary structure of this major glycan which is of the monosialylated and monofucosylated biantennary N ‐acetyllactosaminic type.