Premium
Enzymatic transfer of galactosyl phosphate from UDP‐galactose to UDP‐ N ‐acetylglucosamine
Author(s) -
Nakanishi Yasuo,
Otsu Kaoru,
Suzuki Sakaru
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80332-9
Subject(s) - enzyme , galactose , biochemistry , acetylglucosamine , chemistry , glycoprotein , phosphotransferase , n acetylglucosamine , mannose , transferase
The microsomal fraction of hen oviduct homogenate has been shown to contain an enzyme capable of catalyzing a transfer of galactosyl phosphate from UDP‐galactose to UDP‐ N ‐acetylglucosamine. The product was isolated and identified as UDP‐ N ‐acetylglucosamine‐6‐phosphogalactose, the same compound as that found as a normal constituent in hen oviduct. The enzyme is analogous in reaction type to UDP‐ N ‐acetylglucosamine: glycoprotein N ‐acetylglucosamine‐1‐phosphotransferase (the enzyme responsible for introducing the recognition marker of newly synthesized lysosomal enzymes), which suggests that the galactosyl phosphotransferase is involved in galactose 1‐phosphate transfer to N ‐acetylglucosamine residues of a newly synthesized glycoproteins.