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Methylation of elongation factor 1α in mouse 3T3B and 3T3BSV40 cells
Author(s) -
Coppard N.J.,
Clark B.F.C.,
Cramer F.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80311-1
Subject(s) - elongation , elongation factor , chemistry , microbiology and biotechnology , eukaryotic translation elongation factor 1 alpha 1 , biophysics , biology , biochemistry , materials science , gene , ribosome , rna , metallurgy , ultimate tensile strength
Two‐dimensional gel electrophoretic (NEPHGE) analysis of proteins from mouse 3T3B and 3T3B/SV40 cells labelled with [ methyl ‐ 3 H]methionine in the presence of cycloheximide have revealed that the elongation factor 1α (EF‐1α) in these cells is methylated and that the extent of methylation is higher in the SV40 transformed cell type. It is suggested that methylation may account for differences in growth properties for the different cell types.