Premium
Characterization of a cDNA clone for a rare mRNA modulated by ovariectomy in mammary carcinomas
Author(s) -
Nakhasi Hira L.,
Daruwalla Katie
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80302-0
Subject(s) - complementary dna , messenger rna , clone (java method) , mammary gland , microbiology and biotechnology , chemistry , biology , dna , biochemistry , cancer , gene , genetics , breast cancer
A complementary DNA (cDNA) clone (p13) for a rare mRNA was isolated from a cDNA library generated from total polyA + RNA of 14‐day lactating rat mammary gland. In vitro translation of the positively selected mRNA from p13 cDNA revealed on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS—PAGE) a polypeptide of 24 kDa. The p13 cDNA clone hybridized on northern blots predominantly to ∼1100 base size RNA and weakly to ∼3800 base size RNA from lactating mammary gland. It hybridized only to ∼3800 base size RNA from rat liver. Southern blot analysis of genomic DNA showed differences in gene organization in mammary gland and liver. The mRNA level for the 24 kDa polypeptide was higher in 7–12 DMBA‐induced tumor and lower in the MTW 9 carcinoma as compared to lactating mammary gland. After ovariectomy, the mRNA level in mid pregnant gland increased but was reduced in the 7–12 DMBA tumors.