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Chick retinal pigment epithelium
Author(s) -
Koh Shay-Whey M.,
Chader Gerald J.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80301-9
Subject(s) - cimetidine , histamine , adenylate kinase , histamine h2 receptor , pyrilamine , cyclase , intracellular , receptor , chemistry , histamine receptor , antagonist , epithelium , retinal pigment epithelium , medicine , endocrinology , microbiology and biotechnology , biology , retinal , biochemistry , genetics
Histamine elevates the intracellular cyclic AMP levels in cultured embryonic chick retinal pigment epithelium. The half‐maximal activity is 6 × 10 −6 M. The effect of histamine is mediated by H 2 receptors, i.e., inhibited by the H 2 antagonist cimetidine and not affected by the H 1 antagonists diphenhydramine and pyrilamine. The inhibition constant ( K 1 ) of cimetidine is 1.3 × 10 −8 M. Thus, the present system offers the opportunity of studying the nature of coupling between histamine receptors and adenylate cyclase under controlled conditions in a homogeneous cell type.