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Low‐energy Mg 2+ ‐induced temperature transitions in liver microsomes
Author(s) -
Akhrem A.A.,
Kisel M.A.,
Lunevich A.Ya.,
Bokut S.B.,
Andrianov V.T.,
Kiselev P.A.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80295-6
Subject(s) - isothermal microcalorimetry , thermotropic crystal , pyrene , chemistry , microsome , cytochrome , fluorescence , activation energy , enzyme , phase (matter) , biochemistry , organic chemistry , liquid crystalline , thermodynamics , physics , quantum mechanics , enthalpy
The differential scanning microcalorimetry and fluorescence methods, using probes ANS and pyrene, have been employed to study thermotropic behaviour of rat liver microsomes in the presence and absence of Mg 2+ . Addition of Mg 2+ yields three partially reversible phase transitions at 18, 27 and 32°C, respectively. A character of Mg 2+ ‐induced rearrangements in a membrane and their relation to a catalytic function of a cytochrome P‐450‐dependent enzymatic system discussed.