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O 2 and H 2 O are each the source of one O in NO − 2 produced from NH 3 by Nitrosomonas : 15 N‐NMR evidence
Author(s) -
Andersson Kristoffer K.,
Hooper Alan B.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80292-0
Subject(s) - chemistry , ammonia , oxygen , nitrite , oxygen 18 , nitrate , analytical chemistry (journal) , isotopes of oxygen , nuclear chemistry , inorganic chemistry , environmental chemistry , organic chemistry
The exchange of 18 O between H 2 18 O and exogeneously added 15 N 16 O − 2 which occurs during oxidation of ammonia by Nitrosomonas is shown to occur one oxygen at a time. Conditions in which the exchange is diminished (notably the presence of 14 NO – 2 and CCCP) allowed demonstration that water and dioxygen are each the source of one oxygen in nitrite produced from 15 NH 3 . The nitrate produced in the presence of 18 O 2 consisted of 67 and 0% 15 N 18 O 16 O − and 15 N 18 O 18 O − , respectively. Analysis was made using the 18 O‐isotope shift in 15 N‐NMR.