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Analysis of erythrocyte membrane proteins from dystrophic hamsters
Author(s) -
Pimplikar S.W.,
Malhotra S.K.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80265-8
Subject(s) - erythrocyte membrane , membrane protein , chemistry , microbiology and biotechnology , biochemistry , biology , membrane
To search for potentially mutant proteins, we have investigated erythrocyte ghost proteins from normal and dystrophic hamster by two‐dimensional gel electrophoresis. No significant differences are observed between dystrophic and normal erythrocytes in their peptide patterns on SDS‐polyacrylamide gel electrophoresis while on two‐dimensional gels a protein spot of approximate M r 20000 with an approximate isoelectric point of 4.5 is found in erythrocytes from dystrophic animals and is consistently absent in normal erythrocytes. A large population of erythrocyte (60%) from dystrophic hamsters shows distorted shape as visualized by scanning electron microscopy. The nature of this protein and its relevance in hamster muscular dystrophy are at present not known.