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Short‐term disappearance of muscarinic cell surface receptors in carbachol‐induced desensitization
Author(s) -
Maloteaux Jean-M.,
Gossuin Annie,
Pauwels Petrus J.,
Laduron Pierre M.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80257-9
Subject(s) - carbachol , muscarinic acetylcholine receptor , desensitization (medicine) , receptor , stimulation , agonist , chemistry , biophysics , homologous desensitization , endocrinology , muscarinic acetylcholine receptor m3 , cell , medicine , microbiology and biotechnology , biology , biochemistry
A rapid carbachol‐induced disappearance of muscarinic cell surface receptors was shown using [ 3 H]methyl scopolamine as ligand on intact 108CC15 hybrid cells or rat cerebellar cells. This phenomenon is temperature‐dependent, correlated to agonist stimulation and reversible. In these short time periods (⩽30 min), no change was observed in the total receptor amount measured on membrane preparations. This disappearance of cell surface receptors could represent the first event in cell desensitization which could be followed by receptor recycling in physiological conditions or by receptor degradation if the stimulation by agonists persists, as in long‐term regulation.