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Titration of the binding sites for the oligomycin‐sensitivity conferring protein in beef heart submitochondrial particles
Author(s) -
Dupuis Alain,
Satre Michel,
Vignais Pierre V.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80256-7
Subject(s) - submitochondrial particle , oligomycin , atp hydrolysis , titration , chemistry , atpase , biochemistry , binding site , binding protein , mitochondrion , enzyme , inorganic chemistry , gene
The binding parameters of the oligomycin‐sensitivity conferring protein (OSCP) in inside‐out particles from beef heart mitochondria have been tested by means of two assays, the oligomycin‐sensitive ATP—P i exchange, and the oligomycin‐sensitive ATP hydrolysis. The total number of OSCP binding sites in A particles was equal to 220 pmol/mg particle protein. Each mole of ATPase active site was able to bind 1.1±0.5 mol OSCP with K d 1.7 nM.