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Human liver RNA‐programmed in vitro synthesis of a polypeptide related to human apolipoprotein B
Author(s) -
Olofsson Sven-Olof,
Elias Per,
Boström Kristina,
Lundholm Kent,
Norfeldt Per-Ingvar,
Wiklund Olov,
Fager Gunnar,
Bondjers Göran
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80249-x
Subject(s) - apolipoprotein b , antiserum , in vitro , human liver , rna , translation (biology) , microbiology and biotechnology , messenger rna , biochemistry , protein biosynthesis , chemistry , biology , antibody , gene , immunology , cholesterol
In an in vitro synthesizing system programmed with RNA from human liver a polypeptide with an estimated M r of 80000 (80 kDa)±1400 (mean±SD, n =5) was synthesized. This polypeptide could be precipitated with antiserum to a narrow density cut of LDL ( d =1.030‐1.055) or antiserum against the high‐ M r form of apoB (apoB 100 [4]). The synthesized protein is immunologically related to a 75 kDa protein isolated from LDL. We suggest that the 80 kDa protein represents a primary translation product of apoB synthesized in human liver.