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Differential effect of sulphur deficiency on the composition of the aminoacyl‐tRNA and free amino acid pools of the developing pea seed
Author(s) -
Macnicol P.K.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80247-6
Subject(s) - amino acid , methionine , asparagine , biochemistry , cysteine , aminoacyl trna synthetase , arginine , pisum , transfer rna , sulfur , protein biosynthesis , storage protein , biology , chemistry , rna , enzyme , gene , organic chemistry
Both aminoacyl‐tRNA and free amino acids were extracted from cotyledons of Pisum sativum L., cv. Greenfeast grown under either sulphur‐deficient or control conditions, at a stage of active synthesis and accumulation of storage proteins. Sulphur deficiency severely perturbed the composition of the free amino acid pool, causing a marked decrease in the relative level of cysteine but no change in methionine, as well as large increases in arginine, asparagine and other amino acids. On the other hand, the proportions of both sulphur and non‐sulphur amino acids in the aminoacyl‐tRNA pool were unaltered. This lack of a specific effect of the deficiency on the sulphur amino acids of the aminoacyl pool makes it unlikely that the altered pattern of storage protein synthesis and accumulation is due to unavailability of these amino acids.