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Fluorescence associated with the type 3 copper center of laccase
Author(s) -
Wynn R.Max,
Sarkar Hemanta K.,
Holwerda Robert A.,
Knaff David B.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80240-3
Subject(s) - laccase , fluorescence , phosphorescence , polyporus , copper , photochemistry , chemistry , materials science , analytical chemistry (journal) , enzyme , organic chemistry , optics , physics , biochemistry
Laccases isolated from the lacquer tree Rhus vernicifera and the fungus Polyporus versicolor show fluorescence emission near 420 nm and phosphorescence emission in the 440–465 nm region. The fluorescence and phosphorescence excitation spectra for both laccases show maxima in the 315–330 nm range, a spectral region corresponding to the absorbance maxima for the type 3 binuclear Cu centers of the two enzymes. Additional evidence is presented for the association of the newly discovered emissions with the type 3 Cu centers of the two laccases.