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Structural study of translating 70 S ribosomes from Escherichia coli
Author(s) -
Vasiliev V.D.,
Selivanova O.M.,
Baranov V.I.,
Spirin A.S.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80232-4
Subject(s) - ribosome , protein subunit , escherichia coli , chromosomal translocation , ribosomal rna , stalk , electron microscope , translation (biology) , eukaryotic ribosome , crystallography , chemistry , biology , microbiology and biotechnology , biochemistry , biophysics , physics , rna , gene , messenger rna , optics , horticulture
Translating 70 S ribosomes of Escherichia coli either in the pre‐translocation or in the post‐translocation state have been prepared by using the cell‐free translation system in poly(U)—S—S—Sepharose columns [Methods Enzymol. (1979) 59, 382–398]. Electron microscopy study of the preparations has demonstrated that: (1) the mutual orientation of the ribosomal subunits in the translating ribosomes is the same as proposed by Lake for routine 30 S·50 S couples [J. Mol. Biol. (1976) 105, 111–130]; (2) the L7/L12 stalk of the 50 S subunit sticks out from the 70 S particle and does not join the 30 S subunit; (3) pre‐translocation and post‐translocation state ribosomes do not differ in mutual orientation of the subunits and in the position of the L7/L12 stalk, within the limits of electron microscopy resolution.