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Proton‐pump activity of Nitrobacter agilis and Thermus thermophilus cytochrome c oxidases
Author(s) -
Sone N.,
Yanagita Y.,
Hon-Nami K.,
Fukumori Y.,
Yamanaka T.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80228-2
Subject(s) - thermus thermophilus , cytochrome , thermus , biochemistry , cytochrome c oxidase , chemistry , cytochrome c , enzyme , biology , thermophile , mitochondrion , escherichia coli , gene
The purified cytochrome c oxidases from Nitrobacter agilis and Thermus thermophilus were reconstituted into phospholipid vesicles and their H + pumping activity upon addition of ferrocytochrome c was examined. Thermus cytochrome c oxidase pumped H + , while the Nitrobacter enzyme did not. The process of H + movement was successfully simulated.