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Inorganic pyrophosphate‐driven ATP‐synthesis in liposomes containing membrane‐bound inorganic pyrophosphatase and F 0 ‐F 1 complex from Rhodospirillum rubrum
Author(s) -
Nyrén Pål,
Baltscheffsky Margareta
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80223-3
Subject(s) - rhodospirillum rubrum , inorganic pyrophosphatase , oligomycin , pyrophosphate , chemistry , membrane , pyrophosphatase , liposome , enzyme , biochemistry , phosphorylation , atpase
PP 1 driven ATP synthesis has been reconstituted in a liposomal system containing the membrane‐bound energy‐linked PP 1 ase and coupling factor complex, both highly purified from Rhodospirillum rubrum . This energy converting model system was made by mixing both enzyme preparations with an aqueous suspension of sonicated soybean phospholipids and subjecting to a freeze—thaw procedure. In the presence of ADP, Mg 2+ , P 1 and PP 1 the system catalyzed phosphorylation by up to 25 nmol ATP formed X mg protein −1 X min −1 , at 20°C, which was sensitive to uncouplers and inhibitors of phosphorylation such as oligomycin, efrapeptin and N,N ′‐dicyclohexylcarbodiimide.