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Interactions of antibodies against soluble phosphofructokinase with the soluble and particulate enzymes from yeast
Author(s) -
Huse Klaus,
Kopperschläger Gerhard
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80207-5
Subject(s) - phosphofructokinase , yeast , antiserum , antibody , enzyme , biochemistry , chemistry , specific antibody , biology , glycolysis , immunology
Antibodies obtained from rabbits against soluble yeast phosphofructokinase also react with the particulate yeast phosphofructokinase. Their effects on the activity of the soluble enzyme recognized as inactivation or slight activation depend on the specific immune response of an individual animal yielding antisera with different proportions of inactivating and activating antibodies. The availability of particulate phosphofructokinase to complex inactivating antibodies specifically allows a separation of activating and inactivating antibodies from each other by a simple extraction procedure.