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Effect of electron transfer inhibitors on superoxide generation in the cytochrome bc 1 site of the mitochondrial respiratory chain
Author(s) -
Ksenzenko Marina,
Konstantinov Alexander A.,
Khomutov Gennady B.,
Tikhonov Alexander N.,
Ruuge Enno K.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80200-2
Subject(s) - thenoyltrifluoroacetone , submitochondrial particle , respiratory chain , coenzyme q – cytochrome c reductase , superoxide , chemistry , cytochrome c1 , antimycin a , mitochondrial respiratory chain , cytochrome c , cytochrome , mitochondrion , ubiquinol , biochemistry , enzyme , organic chemistry , extraction (chemistry) , solvent extraction
Antimycin, 2‐nonyl‐4‐hydroxyquinoline N ‐oxide and funiculosin induce O ·− 2 generation by submitochondrial particles oxidizing succinate, whereas KCN, mucidin, myxothiazol or 2,3‐dimercaptopropanol inhibit O ·− 2 generation. Thenoyltrifluoroacetone does not induce superoxide production by itself but slightly stimulates the reaction initiated by antimycin. The results indicate that auto‐oxidation of unstable ubisemiquinone formed in centre o of the Q‐cycle generates most of the O ·− 2 radicals in the cytochrome bc 1 ‐site of the mitochondrial respiratory chain.