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Effect of transmethylation‐reaction and increased levels of cAMP on superoxide generation of guinea‐pig macrophages induced with wheat germ agglutinin and phorbor myristate
Author(s) -
Yagawa Katsuro,
Itoh Tokuo,
Tomoda Akio
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80187-2
Subject(s) - transmethylation , wheat germ agglutinin , superoxide , intracellular , chemistry , guinea pig , agglutinin , biochemistry , microbiology and biotechnology , biology , endocrinology , enzyme , lectin , dna , methylation
Superoxide (O 2 − ) generation of guinea‐pig macrophages induced by wheat germ agglutinin (WGA) was suppressed to a great extent by the inhibition of transmethylation with 3′‐deazaadenosine. When macrophages were stimulated with phorbor myristate (PMA) instead of WGA, the suppression of O 2 − generation of macrophages was observed to be slight despite the presence of 3′‐deazaadenosine. These results were confirmed under various conditions. Thus the WGA‐stimulated O 2 − generation of macrophages is probably associated with transmethylation, but the PMA‐stimulated O 2 − generation is not. WGA‐stimulated O 2 − generation of macrophages was also inhibited in the presence of dibutyryl cAMP or prostaglandin E 2 (PGE 2 ), substances that increase intracellular cAMP, but PMA‐stimulated O 2 − generation was only slightly affected by these compounds. These results suggest that the mechanism for O 2 − generation of macrophages caused by WGA is different from that for O 2 − generation caused by PMA.