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Incorporation of radioactive 35 SO 2− 4 into immunoreactive pituitary lutropin
Author(s) -
Rajyalakshmi M.,
Arunasmithasri S.,
Srinivas V.,
Muralidhar K.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80171-9
Subject(s) - trichloroacetic acid , chemistry , hexosamines , in vitro , biochemistry , radiochemistry , glucosamine
The terminal hexosamines of bovine pituitary lutropin are thought to contain a sulfate moiety. In order to test this, a biosynthetic approach was adopted. When rat and buffalo (bovine) pituitaries were incubated with radioactive 35 SO 2− 4 for 2 h in vitro it was observed that radioactivity gets incorporated into trichloroacetic acid‐precipitable proteins. When the radioactive proteins were treated with an anti‐sheep lutropin serum, radoactivity was found in the immunoprecipitate. The incorporation into rat lutropin like material was very marginal while it was very significant in the case of buffalo lutropin.