Premium
Structure of DNA in metaphase chromosomes of mouse fibroblasts
Author(s) -
Belyaev N.D.,
Budker V.G.,
Dubrovskaya V.A.,
Matveeva N.M.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80167-7
Subject(s) - metaphase , chromatin , dna , microbiology and biotechnology , biology , nuclease , chromosome , genetics , gene
We show tht N‐1 in adenine of chromosomal DNA is methylated by treatment of metaphase chromosomes with dimethylsulphate while this is not the case in chromatin. The data on methylation are consistent with those obtained from the experiments with S 1 ‐nuclease treatment of chromatin and chromosomes. This suggests a disarrangement of DNA secondary structure in the metaphase chromosomes.