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Synchronization of nuclear DNA synthesis in cultured Daucus carota L. cells by aphidicolin
Author(s) -
Sala Francesco,
Galli Maria Grazia,
Nielsen Erik,
Magnien Etienne,
Devreux Marcel,
Pedrali-Noy Guido,
Spadari Silvio
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80148-3
Subject(s) - aphidicolin , daucus carota , dna synthesis , nuclear dna , dna , proliferating cell nuclear antigen , population , microbiology and biotechnology , cell cycle , chemistry , biology , cell culture , cell , biochemistry , mitochondrial dna , gene , genetics , botany , demography , sociology
By inhibiting the α‐like DNA polymerase, and therefore nuclear DNA synthesis, aphidicolin induces accumulation of suspension cultured carrot cells at the G 1 /S boundary of the cell cycle. After a 24‐h treatment with the drug the accumulation is complete, affecting all the cycling cells (95% of the population). Upon removal of the inhibitor, all cycling cells immediately resume nuclear DNA synthesis and move synchronously throughout the S‐phase.