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Specific binding of DCCD to reaction centers of the photosynthetic bacterium Rhodospirillum rubrum and its effect of certain photosynthetic reactions
Author(s) -
Zürrer Hans,
Snozzi Mario,
Bachofen Reinhard
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80137-9
Subject(s) - rhodospirillum rubrum , photosynthetic reaction centre , photosynthesis , chemistry , electron transport chain , carbodiimide , rhodospirillaceae , photochemistry , purple bacteria , membrane , protein subunit , biophysics , biochemistry , biology , enzyme , gene
DCCD binds strongly to chromatophores and reaction centers of the photosynthetic bacterium Rhodospirillum rubrum . Analysis by gel electrophoresis reveals a highly specific binding to subunit M of the reaction center. The binding of the hydrophilic carbodiimide EDC to reaction centers or membranes is weak. Binding of up to 4 DCCD molecules/M subunit has no effect on primary electron transport. With membrane levels of 500 μM DCCD, ATP synthesis was inhibited to 50% but had no effect on the function of the reaction center, the electron transport or the formation of the Δ pH and ΔΨ.